New Application note: Vitamin D analysis

Chromatography

We provide optimal and reliable solutions for your laboratory work in sample preparation, method development and routine analysis.

Analysis of 25-Hydroxyvitamin D in serum by automated sample cleanup and LC-MS/MS

For the analysis of 25-hydroxyvitamin D, a precursor of vitamin D, the combination of solid-phase extraction (SPE) with subsequent HPLC-MS/MS analysis is key to success. Due to the high number of samples in clinical diagnostics, an automated sample preparation is needed. For this purpose, MACHEREY-NAGEL has developed an automated sample preparation in 96-well format on an Eppendorf epMotion® 5075vt robot. CHROMABOND® HLB proved to be ideal as sorbent for analyte enrichment. High recoveries and good reproducibilities could be achieved.

Application note

All information in one document

Find all method details for sample preparation and HPLC analysis in a new compact application note.

Chroma news

Featured products

Solid phase extraction

CHROMABOND® Multi 96 HLB

CHROMABOND® SPE multi 96‑well plates are the choice for high‑throughput (HTP) solid phase extraction with automated robot systems. CHROMABOND® HLB sorbent, a hydrophilic-lipophilic balanced copolymer, is ideal for a wide range of analyte polarities.

HPLC

NUCLEOSHELL® Biphenyl

Hydrophobic, core-shell-based phase with alternative selectivity compared to classical C18 modifications. Due to two retention mechanisms, π-π and hydrophobic interactions, a better retention of aromatic and unsaturated substances is possible.

Vials and caps

An ideal combination

1. Screw neck vial, N 9, 1.5 mL, label, amber, silanized
Benefits: UV-protected, low adsorption
2. Screw closure, N 9, PP, yellow, Silicone white/PTFE red, 1.0 mm