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Method Transfer Considerations for LC — From the Desk of Paul Connolly

From the Desk of

Paul Connolly, Liquid Chromatography Business Manager

Method Transfer Considerations for LC

Transferring methods between liquid chromatographic instruments can pose many challenges due to the number of variables to understand and control. Unless the instruments are identical in design and setup, certain considerations need to be made. Methods transferred between different makes and models, or even the same make and model with a different configuration, will need to be adjusted to obtain similar chromatographic results. Restek wants to make this easier for you with our Pro EZLC Method Translator

Transferring methods between liquid chromatographic instruments can pose many challenges due to the number of variables to understand and control. Unless the instruments are identical in design and setup, certain considerations need to be made. Methods transferred between different makes and models, or even the same make and model with a different configuration, will need to be adjusted to obtain similar chromatographic results. Restek wants to make this easier for you with our Pro EZLC Method Translator

Understanding your system’s gradient delay volume (aka “dwell volume”), temperature and heating type, and extra-column volume are critical when transferring a method from one system to another. It is not enough to assume that going from one UHPLC to another UHPLC and using the same column will generate the same chromatography. Even differences in an instrument’s column heating technology can affect the chromatography. LC column ovens vary and apply heat differently. Some systems preheat the mobile phase and some heat the column. Even when set at the same temperature, their net effect on the separation can vary.

The dwell volume and extra-column volume considerations can be calculated, and adjustments to your method can compensate for these variations. Our Pro EZLC Method Translator allows you to input your differing dwell and extra-column volumes and provides advice on how to assess these variables on your system. Consulting your instrument manuals or inquiring with your instrument manufacturer is a good place to start to understand your configuration, specifications, and volumes. Also, you will need to understand the volume of any tubing or other components that you may have changed since your instrument was installed. Once these volumes are calculated and entered into the Pro EZLC Method Translator, the program will advise you on how to adjust your gradient parameters to achieve a similar chromatogram on both of your systems.

Pro EZLC method translator helps you quickly assess results for different column options from your desk and be certain of method performance before starting in the lab. So, have a look and see how you can simplify method transfer today:

Sincerely,

Paul Connolly

Liquid Chromatography Business Manager